Recent glimpses of the elusive spindle matrix.
نویسندگان
چکیده
A stationary spindle matrix has been proposed on theoretical grounds to help mediate force production at the mitotic spindle. Direct molecular evidence for the existence of such a matrix has the potential to profoundly influence our view of the molecular mechanisms leading to chromosome segregation during mitosis. Three recent papers suggest that the spindle matrix may be more than a theoretical idea.
منابع مشابه
The spindle matrix through the cell cycle in Drosophila.
A spindle matrix has long been proposed to provide structural support for counterbalancing force production and a substrate for essential mitotic factors. For years the molecular identity of such a structure remained elusive. Recently a complex of nuclear proteins that reorganize into a spindle-like structure during prophase through metaphase that shows characteristics of a spindle matrix has b...
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A spindle matrix has been proposed to help organize and stabilize the microtubule spindle during mitosis, though molecular evidence corroborating its existence has been elusive. In Drosophila, we have cloned and characterized a novel nuclear protein, skeletor, that we propose is part of a macromolecular complex forming such a spindle matrix. Skeletor antibody staining shows that skeletor is ass...
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The mitotic spindle is a microtubular assembly required for chromosome segregation during mitosis. Additionally, a spindle matrix has long been proposed to assist this process, but its nature has remained elusive. By combining live-cell imaging with laser microsurgery, fluorescence recovery after photobleaching, and fluorescence correlation spectroscopy in Drosophila melanogaster S2 cells, we u...
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Despite theoretical and physical studies implying that cell-extracellular matrix adhesion geometry governs the orientation of the cell division axis, the molecular mechanisms that translate interphase adhesion geometry to the mitotic spindle orientation remain elusive. Here, we show that the cellular edge retraction during mitotic cell rounding correlates with the spindle axis. At the onset of ...
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Background: The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. The zona pellucid (ZP) is a dynamic matrix composed of filaments with the properties that might reflect the history of oocyte cytoplasmic maturation. Since, spindle and ZP in living oocytes are highly birefringent, their structures can be viewed noninvasively by using a P...
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عنوان ژورنال:
- Cell cycle
دوره 1 5 شماره
صفحات -
تاریخ انتشار 2002